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杂交法和thErmo法计算tm的差异

The melting temperature of an oligonucleotide is calculated with three methods: 1)Thermodynamic Tm: The Tm is calculated using the nearest-neighbor thermodynamic values method (Breslauer et al). This method is less accurate for...

The melting temperature of an oligonucleotide is calculated with three methods: 1)Thermodynamic Tm: The Tm is calculated using the nearest-neighbor thermodynamic values method (Breslauer et al). This method is less accurate for...

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